Study of the Polymorphism of ISSR Markers in Spined Loaches of the Genus Cobitis (Cobitidae) in Connection with Problems of Differentiation of the Species Involved in the Formation of Polyploid Forms of Hybrid Origin and Determination of Their Taxonomic Status

Abstract We have analyzed the polymorphism of ISSR fragments DNA by polymerase chain reaction in spined loaches for first time. The analysis has made it possible to successfully differentiate genomes, which were presumably involved formation polyploid forms hybrid origin: Cobitis taenia ; from Danube River basin, are diagnosed as C. elongatoides tanaitica Don basin and Lake Sinoe, also assigned species a number authors. following conclusions been based on comparative morphological studies previously undifferentiated forms: (1) Central Europe with karyotype 2n = 50, NF 96 correspond diagnosis this differs closely related origin dorsal fin front base pelvic fins, well presence spot lower half caudal base; (2) Sinoe other populations 86 nonconspecific should apparently be ranked an independent species, megaspila .